issue contents
January 2021 issue
research communications
Open access
Three crystals of MHF and its complex with FANCM were obtained, and structural analysis revealed not only their structures but the unexpected release of FANCM from MHF. The biochemical stability of the FANCM–MHF complex was analyzed and it was found that an oxidative environment and organic solvent promote the aggregation and dissociation of the complex.
Protein crystallization and X-ray data collection of the N-terminal Solanaceae domain of the Sw-5b NLR immune receptor are described.
The crystal structure of human V-1 in the apo form was determined at 2.3 Å resolution. The results demonstrate that V-1 is a constitutively active inhibitor of actin-capping protein.
PDB reference: human V-1, apo form, 7df7
methods communications
Yeast surface display was applied to a library of hematopoietic progenitor kinase 1 (HPK1) kinase domain variants in order to select those with an improved expression level and solubility. The HPK1 variant with the most improved properties contained two mutations and crystallized readily in complex with several small-molecule inhibitors.
PDB reference: HPK1, complex with inhibitor, 7kac
ISDSB
Open access
An improved, fully automated protein crystallization and monitoring system, PXS2, can perform crystallization using smaller protein samples than the previous version. In addition, PXS2 can be used for membrane-protein crystallization using the bicelle and LCP methods.