Figure 4
Active site of R. felis acetoacetyl-CoA reductase. The catalytic triad of R. felis (Ser135, Tyr148 and Lys152) found in helix α6 and the connecting loop to β5 is shown as sticks (magenta, top panel). Alignment of R. felis acetoacetyl-CoA reductase with NADP+-bound B. pseudomallei acetoacetyl-CoA reductase (green; catalytic triad shown as sticks) demonstrates necessary rearrangements to the active site, including movement of Ser135 and reorientation of Lys152, to accommodate substrates (lower panel). |