Figure 2
Determination of the Cys528 DeStreak modification of human CRM1 with 2-mercaptoethanol (BME) using mass-spectrometric analysis. (a) List of possible fragment ions of the Cys528–BME-containing peptide [DLLGLC(+75.99)EQK] with detected y ions in blue and b ions in red. (b) Representative fragmentation spectrum (1 of 15) of the respective precursor ion with an m/z of 547.765. RT, retention time. |