 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 5]](rl5195fig5mag.jpg)
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Figure 5
Biophysical characterization of PaaR21–57 and PaaR21–66. (a) Analytical SEC profiles of PaaR21–57 (blue) and PaaR21–66 (red) at 5 mg ml−1. The elution volumes of the molecular-weight standards (bovine γ-globulin, 158 000 Da; chicken ovalbumin, 44 000 Da; horse myoglobin, 17 000 Da; vitamin B12, 1350 Da) are plotted as white squares. The elution volumes of PaaR21–57 (marked as a red square) and PaaR21–66 (blue square) give molecular-weight estimates of 4.8 and 6.7 kDa, respectively. (b) CD spectra of PaaR21–57 (blue) and PaaR21–66 (red) measured at 25°C at a concentration of 0.3 mg ml−1. (c) Thermal unfolding of PaaR21–57 (blue) and PaaR21–66 (red) followed by CD spectroscopy at 222 nm and 0.3 mg ml−1 (triangles) or 0.03 mg ml−1 (circles). |
![[Figure 5]](rl5195fig5.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
