Structural characterization of DynU16, a START/Bet v1-like protein involved in dynemicin biosynthesis

The crystal structure of DynU16, a protein identified in the dynemicin-biosynthetic gene cluster of Micromonospora chersina, was determined using iodide phasing and reveals a di-domain helix-grip fold.

Structural characterization of human peptidyl-arginine deiminase type III by X-ray crystallography

Peptidyl-arginine deiminases (PADs) catalyse the post-translational modification of arginine residues in numerous proteins. The human type III isoform (PAD3) is specifically expressed in skin tissue and is required for normal skin and hair formation. The structure of human PAD3 has been determined at 2.8 Å resolution, giving insight into the substrate specificity of the enzyme.

Crystal structure of the complex of DNA with the C-terminal domain of TYE7 from Saccharomyces cerevisiae

TYE7 has abundant physiological functions. Here a complex of DNA with the C-terminal domain of TYE7 from Saccharomyces cerevisiae has been determined. Detailed structural analysis shows that Arg193 in TYE7 is unusual compared with Arg193 in other similar structures.

Crystal structures of human coronavirus NL63 main protease at different pH values

Four crystal structures of human coronavirus NL63 main protease (M^pro) in the apo form at different pH values are reported at resolutions of up to 1.78 Å. Comparison with M^pro from other human betacoronaviruses such as SARS-CoV-2 and SARS-CoV reveals common and distinct structural features in the different genera and extends knowledge of the diversity, function and evolution of coronaviruses.

Microcrystal preparation for serial femtosecond X-ray crystallography of bacterial copper amine oxidase

Microcrystals of bacterial copper amine oxidase were prepared by combining micro-seeding and batch crystallization, and were used to determine a radiation-damage-free, nonfrozen structure by serial femtosecond X-ray crystallography.

Crystal structure determination of the armadillo repeat domain of Drosophila SARM1 using MIRAS phasing

The cystallization and structure determination of the armadillo repeat domain of Drosophila SARM1 are described. The phase problem was solved using the MIRAS technique with autoSHARP, combining diffraction data from native, selenomethionine-labelled and bromide-soaked crystals.

Nanobody-aided crystallization of the transcription regulator PaaR2 from Escherichia coli O157:H7

Native mass spectrometry was used to screen for nanobodies that allowed crystallization of the transcription regulator PaaR2.