research communications
Joint X-ray/neutron structure of Lentinus similis AA9_A at room temperature
aDepartment of Chemistry, University of Copenhagen, Universitetsparken 5, 2100 Copenhagen, Denmark, bDepartment of Molecular and Structural Biochemistry, North Carolina State University, Campus Box 7622, Raleigh, NC 27695, USA, and cNeutron Scattering Division, Oak Ridge National Laboratory, PO Box 2008, Oak Ridge, TN 37831, USA
*Correspondence e-mail: fmeille@ncsu.edu
Lytic polysaccharide monooxygenases (LPMOs) are copper metalloenzymes which cleave Lentinus similis family AA9 isoform A (LsAA9_A) has been extensively studied as a model system because its activity towards smaller soluble saccharide substrates has allowed detailed structural characterization of its interaction with a variety of substrates by X-ray crystallography at high resolution. Here, the joint X-ray/neutron room-temperature crystallographic structure of carbohydrate-free LsAA9_A in the copper(II) resting state refined against X-ray and neutron data at 2.1 and 2.8 Å resolution, respectively, is presented. The results provide an experimental determination of the protonation states of the copper(II)-coordinating residues and second-shell residues in LsAA9_A, paving the way for future neutron crystallographic studies of LPMO–carbohydrate complexes.
oxidatively and are important in pathogen biology, carbon cycling and biotechnology. TheKeywords: lytic polysaccharide monooxygenases; Lentinus similis AA9_A; copper metalloenzymes; protonation states; neutron crystallography.
PDB reference: Neutron of Lentinus similis AA9_A at room temperature, 8e1w
1. Introduction
Copper-containing lytic polysaccharide monooxygenases (LPMOs) are a class of metalloenzymes that have recently been brought to the forefront of research advancing renewable energy and the conversion of biomass to value-added chemicals (Johansen, 2016), as well as having increasingly recognized biological functions in pathogenesis (Vandhana et al., 2022). They are classified as auxiliary activities (AA) families AA9–AA11 and AA13–AA17 in the Carbohydrate Active enZymes (CAZy) database (Levasseur et al., 2013; Drula et al., 2022). Fungal LPMOs are some of many enzymes secreted by fungi to break down plant matter, namely cellulose, and release glucose. LPMOs randomly oxidize recalcitrant crystalline cellulose, disrupting local crystallinity and creating new cellodextrin chain ends that serve as additional starting points for the endo- and exo-activities of glycoside (GHs; Agger et al., 2014). While LPMOs were first described as monooxygenases, binding molecular dioxygen as a co-substrate, it is now established that LPMOs also have peroxidase/peroxygenase activity (Bissaro et al., 2017). The monooxygenase reaction requires molecular oxygen, two protons and two single-electron transfers from either small-molecule reductants or electron-donating proteins such as cellobiose dehydrogenases that are co-secreted by fungi (Fig. 1). Mechanistic details, including the source of protons and the chemical nature of oxygen-activated reaction intermediates, for both the O2-based and H2O2-based mechanisms remain to be elucidated in order to understand how these enzymes carry out carbohydrate oxidation.
Neutron protein crystallography is a powerful tool for investigating protein chemistry because it directly locates H-atom positions in a protein structure (Schröder et al., 2018). Early X-ray and neutron crystallographic studies of LPMOs from the AA9 family have focused on understanding the activation of O2 by Neurospora crassa LPMO 9D (NcAA9_D) in the absence of substrate (Bodenheimer et al., 2017; O'Dell, Swartz et al., 2017; O'Dell, Agarwal et al., 2017; Schröder et al., 2021, 2022). In addition to the direct determination of protonation states, these structures have revealed the geometry and the chemical nature of the initial Cu—O2− and Cu—O2H intermediates. These results are important to understand the activation of O2 (Fig. 1f). However, elucidation of the mechanism of carbohydrate oxidation requires the trapping of early intermediates in an LPMO–carbohydrate complex (Fig. 1b). The insolubility of the substrates of NcAA9_D has so far prevented the investigation of LPMO–O2–carbohydrate complexes. Later, LsAA9_A was demonstrated to be active towards soluble and the first structures of an LPMO enzyme in complex with short soluble were reported (Frandsen et al., 2016; Simmons et al., 2017). LsAA9_A has now been used extensively as a model LPMO, including high-resolution crystallographic studies to determine the protonation state of key histidine residues (Banerjee et al., 2022) and detailed studies (Tandrup et al., 2022). Furthermore, the dependence of LsAA9_A on H2O2 has been established and a novel twist on the LPMO mechanism has been suggested, at least for this specific enzyme (Brander et al., 2021). Therefore, LsAA9_A opens the opportunity to analyze substrate-bound enzyme complexes and the activation of H2O2 using neutron crystallography.
Here, we report the neutron structure of carbohydrate-free LsAA9_A in the copper(II) resting state. The protonation states of catalytic residues at and around the copper center are determined.
2. Materials and methods
2.1. Protein purification and crystal growth
The fungal enzyme LsAA9_A was expressed in Aspergillus oryzae, purified and deglycosylated with endoglycosidase H as described previously (Frandsen et al., 2016; Simmons et al., 2017; Tandrup et al., 2020). The protein sample was pre-incubated with an equimolar amount of copper(II) acetate for 1 h at 4°C prior to crystallization setup. Crystals of LsAA9_A were grown via sitting-drop vapor diffusion by adapting previously reported protocols (Frandsen et al., 2016). For neutron crystallography, crystals were grown in a nine-well glass-plate and sandwich-box setup (Hampton Research) at 10°C. The sitting drops were equilibrated against 50 ml reservoir solution consisting of 3.0 M NaCl, 0.1 M citric acid pH 3.5. Large crystals grew from 100 µl sitting drops prepared at a protein concentration ranging from 16 to 20 mg ml−1 in 1.3 M NaCl, 0.1 M citric acid pH 3.5 (Fig. 2).
2.2. Neutron and X-ray data collection at room temperature
Crystals were mounted in thin-walled quartz capillaries (Hampton Research) using hydrogenated crystallization buffer at pH 3.5 from the sandwich box. Excess buffer was removed and plugs of 100 mM deuterated citric acid buffer at pD 5.5 (pH electrode reading 5.1) containing 3 M NaCl were placed on both sides of the crystal prior to sealing the capillaries to vapor-exchange the crystal solvent water molecules and labile protein H atoms to D2O molecules and D atoms, respectively. The pH was increased from 3.5 to 5.5 to prevent disorder of the histidine brace (Frandsen et al., 2017). The exchange occurred over two weeks prior to neutron data collection.
Neutron time-of-flight diffraction data were collected at room temperature on the MaNDi instrument at the Spallation Neutron Source (Coates & Sullivan, 2020; Meilleur et al., 2018). An incident neutron wavelength bandpass of 3–5 Å was used. A total of six diffraction patterns with a Δφ of 10° between frames were collected with an exposure of 48 h per frame. Following neutron diffraction data collection, an X-ray data set was collected from the same crystal at room temperature on a microfocus rotating-anode X-ray diffractometer (MicroMax-007 HF, Rigaku). A total of 57 diffraction patterns were collected with a Δφ of 1.0° and an exposure of 45 s per frame.
The neutron data set was reduced using the Mantid package (Arnold et al., 2014) and integrated using three-dimensional profile fitting (Sullivan et al., 2018). The data were wavelength-normalized using LAUENORM from the LAUEGEN suite (Helliwell et al., 1989; Campbell et al., 1998; Arzt et al., 1996). The X-ray data were indexed and integrated using CrysAlisPro (Rigaku, Woodlands, Texas, USA) and scaled and merged with AIMLESS in the CCP4 suite (Evans & Murshudov, 2013; Winn et al., 2011). Data-collection and processing statistics are summarized in Table 1. The data and structure have been deposited in the Protein Data Bank (PDB entry 8e1w; Berman et al., 2000).
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2.3. Structure refinement
Joint X-ray/neutron Phenix software suite (Liebschner et al., 2019) with manual model building performed in Coot (Emsley et al., 2010).
was performed using theThe ligand-free model of LsAA9_A at pH 3.5 (PDB entry 5n04; Frandsen et al., 2017) stripped of the Cu2+ ion and water molecules was used as the starting model. Iterative rounds of and model building were conducted. When the model refined against the X-ray data alone was complete, H and D atoms were generated using the Phenix ReadySet! tool as described by Schröder & Meilleur (2020). The model was further refined against both the X-ray and neutron data sets. are listed in Table 1.
3. Results and discussion
Neutron and X-ray data sets were collected from the same crystal to 2.8 Å and 2.1 Å resolution, respectively. The LsAA9_A model refined jointly against the X-ray and neutron data includes key LPMO structural features at the active site.
3.1. Vapor exchange
Neutron diffraction data collection requires hydrogen to be exchanged for deuterium to increase the signal-to-noise ratio of the data and the visibility of the hydrogen/deuterium positions (Meilleur, 2020). Vapor exchange was performed over a period of two weeks, which is typical of the time used in a neutron protein crystallography experiment to exchange hydrogen for deuterium (O'Dell et al., 2016). The hydrogen–deuterium exchange pattern for the backbone amide groups is presented in Fig. 3. As expected, the outer loops show a high level of exchange, while the inner β-sheets undergo limited exchange. However, the overall exchange is low (35%), indicating that at this low pH longer times are required to achieve higher overall exchange. The pH dependence of the amide hydrogen-exchange rate has previously been examined using mass-spectrometry experiments. These studies suggest that the exchange time required at pH 5.5 to achieve the same level of exchange as at pH 7.5 is 100 times longer (Li et al., 2014). This time scale is challenging to achieve when planning for neutron data collection.
3.2. The histidine brace
As previously described for LsAA9_A, the Cu2+ ion in the joint X-ray/neutron structure presented here is coordinated by the N-terminal amino group and Nδ of His1, Nɛ of His78 and a water molecule in the equatorial position (H2O-eq), while the OH group of Tyr164 and a water molecule are located close to the axial coordination sites. The distances of the copper to the ligands are listed in Table 2.
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The crystals were grown at pH 3.5. At this pH the histidine brace is disordered, with His78 adopting two conformations as reported previously for LsAA9_A (Frandsen et al., 2017). Ordering of the histidine brace requires the pH to be increased. The pH increase is typically achieved by soaking crystals directly in buffer at pH 5.5. Here, to avoid compromising the diffraction quality of the large crystals, the pH was increased by vapor diffusion.
Clear electron and neutron scattering-length densities were observed for His78 coordinated to Cu2+, confirming that vapor pH exchange drove ordering of the histidine brace (Figs. 4a and 4b). No residual Fo − Fc electron density was observed for an alternate conformation of His78 flipped away from the active-site Cu2+. However, residual Fo − Fc electron density indicated that a Cl− ion from the crystallization condition alternatively coordinates the Cu2+ ion instead of His78 as observed in the structure of LsAA9_A previously solved at pH 3.5 (Frandsen et al., 2017; Fig. 4c). The occupancies of His78 and Cl− refined to 0.75 and 0.25, respectively. The N-terminal amino group is neutral (Fig. 4d).
3.3. Active-site waters
The active sites of LPMOs are readily prone to X-ray-induced et al., 2014; Muderspach et al., 2019; Banerjee et al., 2022). At the active site of LPMO, causes disorder (and ultimately the disappearance) of the copper-coordinating water molecules in the copper axial and equatorial positions and other geometrical changes. An advantage of neutron diffraction in the structural characterization of metalloenzymes is the lack of radiation-induced in-beam chemistry (Bodenheimer et al., 2017; Schröder & Meilleur, 2021). While joint against neutron and X-ray data is a standard approach (O'Dell et al., 2016), the structure refined against the X-ray data alone must be carefully examined when planning the joint X-ray/neutron of a For the joint performed here, we collected an X-ray data set on a home source. The X-ray data show clear Fo − Fc electron-density peaks for the copper equatorial and axial water molecules, as well as for the pocket water (Fig. 5). The distances from H2O-eq and H2O-ax to Cu2+ are 2.1 and 2.7 Å, respectively, confirming that the copper ion is in the resting copper(II), and validating the use of the X-ray data for joint (Tandrup et al., 2022).
as shown by studies on families AA9, AA10 and AA13 (Gudmundsson3.4. Residues near the active site: Tyr164 and His147
The LPMO family AA9 active site includes a conserved tyrosine residue. The tyrosine hydroxy group is in the second axial coordination site of the copper, but the Cu–OTyr distance is too long to form a Cu—OTyr bond. Recent analysis of X-ray structures at low X-ray dose have confirmed that on binding oligosaccharide the distance to Tyr164 is shortened (Tandrup et al., 2022). In the structure presented here, Tyr164 is positioned in the axial position of the copper with a Cu–OTyr distance of 2.80 Å. The Tyr164 OH group forms a hydrogen bond to the side-chain carbonyl group of Gln162 with an OTyr164–OGln162 distance of 2.72 Å. An Fo − Fc nuclear map calculated omitting the D atom of the Tyr164 hydroxy group show a clear positive peak at 3.0σ (Fig. 6a). It will be interesting to observe the effect of saccharide binding on this hydrogen bond in future neutron structures.
His147, a conserved second-shell residue, is singly protonated at the NE2 position (Fig. 6b). This confirms that in this conformation His147 remains neutral at low pH (Schröder et al., 2022), as also shown by analysis of high-resolution X-ray structures (Banerjee et al., 2022).
4. Conclusion
The protonation state of key amino acids in the active site of LsAA9_A could be determined despite the modest resolution of the neutron data. For future studies, longer pH exchange times are advised, as a small amount of disorder remained at the histidine brace. Perdeuteration allows diffraction from similar-sized crystals to extend to higher resolution by eliminating the of hydrogen which contributes to the high background. The heterologous expression of LsAA9_A was recently optimized in Escherichia coli, which opens the opportunity to perdeuterate the protein (Hernández-Rollán et al., 2021). LsAA9_A produced in E. coli was crystallized and high-quality X-ray structures were obtained, including a complex with cellotriose (Banerjee et al., 2022; Tandrup et al., 2022). Future neutron structural investigation of LsAA9_A in complex with carbohydrate ligands will be greatly enhanced by the use of perdeuterated protein.
Footnotes
‡Current affiliation: Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads 224, 2800 Kongens Lyngby, Denmark.
Acknowledgements
The authors thank Novozymes for expressing LsAA9_A in A. oryzae and their gift of concentrated culture medium, and Gwyndalyn Phillips for technical support. Protein purification and crystallization experiments were conducted at the Center for Structural Molecular Biology (CSMB), a US Department of Energy Biological and Environmental Research User Facility at Oak Ridge National Laboratory. Neutron diffraction data were collected on BL-11B MaNDi at the Spallation Neutron Source at ORNL, which is sponsored by the Scientific User Facilities Division, Office of Basic Energy Sciences, US Department of Energy. Thanks also to Johan Ø. Ipsen and Katja S. Johansen (University of Copenhagen) for helpful discussion and help with sample preparation.
Funding information
FM acknowledges support from USDA NIFA Hatch 211001. TT and LLL acknowledge financial support from the Novo Nordisk Foundation (HOPE project NNF17SA0027704) and the Danish Council for Independent Research (grant No. 8021-00273B). TT acknowledges the support from The Fulbright Program towards his research stay at Oak Ridge National Laboratory.
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