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Figure 6
The AbTFM protein does not display toxicity upon heterologous expression in bacterial or yeast cells. (a) Growth of E. coli MG1655 carrying an empty vector control (pBAD18-Kn; vector) or plasmids directing the expression of native AbTFM (AbTFM) or of AbTFM with an N-terminal Tat signal peptide (sp-AbTFM) on LB medium with 0.2% glucose or 0.02% or 0.2% L-arabinose to repress (glucose) or induce (L-arabinose) gene expression. (b) Growth of S. cerevisiae K699 transformed with an empty vector control (pRB1438; vector) or an integrative plasmid directing the expression of AbTFM (AbTFM) on DOA-URA plus 2% raffinose medium with 1% glucose or 0.5% or 1% galactose to repress (glucose) or induce (galactose) gene expression.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 79| Part 1| January 2023| Pages 8-16
https://doi.org/10.1107/S2053230X22011414
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