Introduction to the virtual thematic issue on room-temperature biological crystallography

Room-temperature biological crystallography has seen a resurgence in recent years and a collection of articles recently published in IUCrJ, Acta Cryst. D Structural Biology and Acta Cryst. F Structural Biology Communications, have been collected together to produce a virtual special issue at https://journals.iucr.org/special_issues/2022/RT/.

Monomeric crystal structure of the vaccine carrier protein CRM₁₉₇ and implications for vaccine development

The first crystal structure of the widely used vaccine carrier protein CRM₁₉₇ in its monomeric form reveals features underlying its dimerization and vaccine-conjugation reaction.

Structural and enzymatic characterization of the sialidase SiaPG from Porphyromonas gingivalis

The crystal structure of the sialidase SiaPG from Porphyromonas gingivalis, a pathogenic bacterium which causes human periodontal disease, is reported.

A partially open conformation of an androgen receptor ligand-binding domain with drug-resistance mutations

A partially open conformation of the human androgen receptor ligand-binding domain containing four drug-resistance mutations, L702H/H875Y/F877L/T878A, was observed. Of these mutations, both L702H and F877L are important for this conformation, which may affect ligand binding as well as resistance to antagonists.