Figure 2
LcGNAT homologues and their cosubstrate-binding modes. (a) Structural superimposition of LcGNAT with BsPaiA (PDB entry 1tiq), SmGNAT (PDB entry 4e2a), TaPaiA (PDB entry 3k9u) and TvArd1 (PDB entry 4pv6). (b) Crystal structure of LcGNAT displayed with the Ac-CoA or CoA ligands bound to homologues structurally aligned as in (a). Ligands are colour-coded: C atoms correspond to the ribbon colour in (a), N atoms are blue, O atoms are red, S is in yellow and P is in orange. (c) LcGNAT in surface representation displaying residues which correspond to Ac-CoA-binding residues in BsPaiA (PDB entry 1tiq). The Ac-CoA ligand in BsPaiA is shown in stick representation. |