 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](no5205fig2mag.jpg)
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Figure 2
Effect of the M405S mutation on the structure of DvFdhAB. In all representations, the tungsten active site is shown as sticks; the tungsten ion, sulfido group and the U192 Se atom are shown as spheres (light blue, yellow and orange, respectively). (a) The M405S mutation is shown as sticks in the structure of the M405S variant (black). 2Fo − Fc electron-density maps at 1σ are shown as a blue mesh for the side chain of Ser405. The Ser405 Oγ–W distance and the hydrogen bonds established to O2α and O2β from the phosphate groups of MGD1 are shown as black and orange dashed lines, respectively. Distances are shown in Å. (b) The M405S variant (black) in a different orientation. Ser405 and the residues with greater conformational changes (U192, Thr408 and Gln409) are shown as sticks and their respective 2Fo − Fc electron-density maps at 1σ are shown as a blue mesh. (c) A superposition of the M405S (black) and M405A (PDB entry 8cm7; cyan) variants is shown. The mutated residue (Met405) and the residues with greater conformational rearrangement in the M405S variant (U192, Thr408 and Gln409) are shown as sticks. In the M405A variant the U912 side chain could not be modelled and is absent in the deposited structure. (d) A superposition of the M405S variant (black) and the oxidized WT (red; PDB entry 6sdr) is shown in the same orientation as in (c). (e) A superposition of the M405S variant (black) and the reduced WT (green; PDB entry 6sdv) is shown in the same orientation as in (c). (f) A superposition of the M405S (black) and C872A (violet; PDB entry 8cm6) variants is shown in the same orientation as in (c). |
![[Figure 2]](no5205fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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