view article

Figure 5
Effect of the absence of the Met405 side chain on the geometry of the tungsten active site in DvFdhAB. In all segments, the tungsten active site is shown as sticks and the tungsten ion, the S atoms (sulfido ligand and MGDs dithiolene groups) and the U192 Se atom are shown as spheres (light blue, yellow and orange, respectively). (a) A superposition of the M405S variant (black), the oxidized WT (red; PDB entry 6sdr), the formate-reduced WT (green; PDB entry 6sdv) and the C872A variant (violet; PDB entry 8cm6) is shown. The structures were aligned using MGD1, as this cofactor shows the minimal conformational variability between the four structures. (b) The M405S variant (black) is shown. The anomalous map peaks at 3σ are shown as a green mesh for the W and Se atoms.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Follow Acta Cryst. F
Sign up for e-alerts
Follow Acta Cryst. on Twitter
Follow us on facebook
Sign up for RSS feeds