 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](yg5011fig2mag.jpg)
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Figure 2
Brief overview of the negative-staining workflow. Starting from glow-discharged sample-carrier grids with a continuous support film (a), protein in buffer solution is incubated on the grid surface (b). Grids are then washed with sample buffer (c). For AMo and SPT negative staining an on-grid fixation step using 0.15% glutaraldehyde solution is employed to fix the sample (d) before staining using a droplet of staining solution (e). Afterwards, the stain is rapidly dried to create an amorphous film around the sample (f). For a detailed step-by-step guide, see Supplementary Fig. S2. |
![[Figure 2]](yg5011fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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