Crystal structure of N-terminally hexahistidine-tagged Onchocerca volvulus macrophage migration inhibitory factor-1

N-terminally hexahistidine-tagged O. volvulus macrophage migration inhibitory factor-1 has a unique jellyfish-like structure with the prototypical macrophage migration inhibitory factor trimer as the `head' and a C-terminal extension as the `tail'.

Crystal structure of glutamyl-tRNA synthetase from Helicobacter pylori

Persistent H. pylori infection causes gastric ulcers and cancer, and multidrug resistance is increasing globally. The 2.5 Å resolution structure of H. pylori glutamyl-tRNA synthetase was determined as a first step towards rational drug discovery.

Structures of Trichomonas vaginalis macrophage migratory inhibitory factor

The production, crystallization and three crystal structures of macrophage migratory inhibitory factor from T. vaginalis are reported.

Crystal structure of a short-chain de­hydrogenase from Burkholderia cenocepacia J2315 in complex with NADP⁺ and benzoic acid

The structure of B. cenocepacia short-chain dehydrogenase/reductase (SDR) in complex with NADP⁺ and benzoic acid is presented. Analysis of the structure reveals a distinctive active-site architecture, suggesting that this protein is part of the divergent SDR subgroup.

Revisiting sodium phosphotungstate and ammonium molybdate as nonradioactive negative-staining agents for single-particle analysis

The most often used negative stains for single-particle analysis are based on the radioactive element uranium. Here, two stains based on tungsten or molybdenum are re-established as nonradioactive substitutes to allow the use of negative-staining electron microscopy by a broader user base in structural biology projects.