Figure 3
SPT or AMo staining allows assessment of the quality of β-galactosidase preparations similar to UF staining. Representative raw micrographs of β-galactosidase stained with (a) UF, (b) SPT or (c) AMo are shown. Scale bars are 100 nm. The insets show the boxed area at 2.5× magnification. Instances of stain accumulation, commonly associated with protein aggregates, are marked by arrows. |