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Figure 4
UF, SPT and AMo all allow faithful 3D reconstructions of β-galactosidase to be obtained by single-particle analysis. (a) Selected 2D class averages for β-galactosidase obtained from grids prepared by either UF, SPT or AMo negative staining. (b) Surface representation of 3D density maps calculated from these data sets, filtered to 10 Å for comparability. The atomic model of β-galactosidase (PDB entry 6x1q, coloured by individual subunits) was rigid-body fitted to each map. The scale bar is 25 Å. For the complete 3D reconstruction workflow of each condition, see Supplementary Figs. S3, S4 and S5.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 80| Part 12| December 2024| Pages 356-362
https://doi.org/10.1107/S2053230X24011294
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