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Figure 1
Assessment of protein purification. (a) Size-exclusion chromatography indicates that the target protein (72.6 kDa) is monomeric. Masses of molecular-weight standards are indicated at the top. Red lines indicate the pooled fractions, while the four circles correspond to the elution fractions shown in (b). (b) SDS–PAGE of peak Superdex 200 fractions. Several co-purified contaminants are present, including CA2 (25.0 kDa).

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
Volume 81| Part 2| February 2025|
https://doi.org/10.1107/S2053230X25000068
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