Crystallographic analysis of the Escherichia coli tRNA seleno-modification enzyme in complex with tRNA

tRNA 2-selenouridine synthase (SelU) produces a 5-substituted 2-selenouridine at the tRNA wobble position, which can fine-tune translation. Crystals of the SelU–tRNA complex were obtained and the initial structure was determined at 3.10 Å resolution.

The structure of His15 acetamide-modified hen egg-white lysozyme: a nice surprise from an old friend

Chemical modification of lysozyme at His15 using iodoacetamide was investigated structurally. Crystallization of the chemically modified enzyme using a protocol which yields tetragonal crystals gave orthorhombic crystals with similar unit-cell parameters.

Serendipitous high-resolution structure of Escherichia coli carbonic anhydrase 2

Contaminating proteins from the expression source may inadvertently crystallize instead of the target protein of interest. Two novel crystal forms of E. coli carbonic anhydrase 2, as well as a high-resolution (1.43 Å) structure of the enzyme, are reported.

CryoCrane: an open-source GUI for analyzing cryo-EM screening data sets

CryoCrane is a GUI which allows rapid analysis of cryo-EM screening data and supervision of data collections.