A two-in-one expression construct for biophysical and structural studies of the human pregnane X receptor ligand-binding domain, a pharmaceutical and environmental target

Carivenc, C.; Laconde, G.; Blanc, P.; Amblard, M.; Bourguet, W.; Delfosse, V.

doi:10.1107/S2053230X2500069X

Acta Crystallographica Section F
Acta Crystallographica
Section F STRUCTURAL BIOLOGY COMMUNICATIONS

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Figure 1
A two-in-one construct that provides a large quantity of pure PXR_LBD with or without SRC-1. (a) Schematic representation of the primary sequence of the cleavable PXR_LBD-Thb-SRC-1 construct. (b) Gel-filtration elution profiles for the PXR_LBD-Thb-SRC-1 (solid line) and PXR_LBD (dashed line) proteins. The SDS–PAGE inset shows the PXR_LBD samples before (lane nc) and after (lane c) thrombin cleavage. The ladder is labelled in kDa. (c) Thermal unfolding profiles of the PXR_LBD-Thb-SRC-1 (solid line) and PXR_LBD (dashed line) proteins before freezing (black) and after thawing (gray). For the latter, results are shown for samples that were thawed 18 months after purification.

STRUCTURAL BIOLOGY
COMMUNICATIONS

ISSN: 2053-230X

Volume 81| Part 3| March 2025|

https://doi.org/10.1107/S2053230X2500069X

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