 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 2]](pg5096fig2mag.jpg)
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Figure 2
(a) The structure of the SsoGINS tetramer is an approximately twofold-symmetric assembly of two GINS51 subunits (green and cyan) and two GINS23 subunits (magenta and yellow). Both subunit types consist of two subdomains; one is fully α-helical (A) and the other contains β-strands (B). These subdomains occur in reverse order for the two subunit types. The secondary, tertiary and quaternary structures are highly similar to those previously observed for the GINS complexes from the archaeon Thermococcus kodakarensis (b) (PDB entry 3anw; Oyama et al., 2011  ) and the eukaryotic yeast (c) (PDB entry 7z13; Lewis et al., 2022). The structurally conserved core consists of the full GINS23 subunits (yeast Psf2 and Psf3) and the A subdomains of GINS51 (yeast Sld5 and Psf1). The B subdomains of GINS51 (yeast Sld5 and Psf1) occupy variable positions, indicating that this subdomain may be mobile. Structure images were prepared with PyMOL (Schrödinger) and secondary-structure diagrams were prepared with the Google Colab implementation of SSDraw (Chen & Porter, 2023). The SsoGINS51A subdomains uniquely form a significant dimeric interaction. |
![[Figure 2]](pg5096fig2.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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