 | Acta Crystallographica Section F Acta Crystallographica Section F STRUCTURAL BIOLOGY COMMUNICATIONS |
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![[Figure 5]](pg5096fig5mag.jpg)
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Figure 5
Generation of a model for SsoCMG. (a) A model for the structure of the SsoMCM N-terminal domain hexamer bound to SsoGINS and SsoRecJ predicted by AlphaFold-Multimer (Evans et al., 2021  ) is illustrated on the left. The ScCMG portion of PDB entry 7z13 (Lewis et al., 2022) is illustrated on the right and the overlay of the two is shown in the center. The MCM subunits are colored purple, blue, green, yellow, orange and red. The GINS subunits are colored yellow, magenta, green and cyan in the same scheme as the other figures. RecJ/Cdc45 subunits are in salmon and light gray as in the other figures. The MCM–GINS portions are consistent between the two structures. The SsoRecJ subunit interacts with GINS51B as in Fig. 4(a), which places it opposite the MCM ring rather than adjacent as in ScCMG. (b) A model for SsoCMG was generated from the structures in (a) by superimposing one of the SsoGINS51B–RecJ units (green and salmon) on the Psf1B and Cdc45 subunits of ScCMG and regularizing the SsoGINS51 linker. The ATPase domains of SsoMCM were added by superimposing the structure of the nearly full-length SsoMCM hexamer crystal structure (PDB entry 6mii; Meagher et al., 2019). Superpositions and figures were prepared in PyMOL (Schrödinger). |
![[Figure 5]](pg5096fig5.jpg)
| STRUCTURAL BIOLOGY COMMUNICATIONS |
ISSN: 2053-230X
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