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Figure 3
Product-length pocket and floors. (a) The three floors in Rv2173: floor 1 in light pink, floor 2 in orange and floor 3 in blue with Tyr198 in lime. (b) Close-up showing the floor residues as a surface. In both panels to mark the allylic DMAPP site, DMAPP is shown as magenta sticks, with metal ions as green spheres. The FPP (C15) bound in as the substrate in this site from a superposition with PDB entry 2e8t is shown in dark grey. The GGPP (C20) from PDB entry 2e8v as the product in the IPP site is shown as light grey sticks. GGPP comes into close contact with the floor residues, suggesting that a smaller size (C10 or C15) product would be preferred. |