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Figure 3
DNA-ligation activity of different DNA breaks by T4-like DNA ligase enzymes. The Nick substrate contained a break on a single strand of the DNA duplex. The overhang substrate contained a cohesive double-stranded break with a four base-pair overhang, the TA + PEG substrate contained a cohesive double-stranded break with a single T–A overhang and included 10%(w/v) PEG 3350, and the Blunt + PEG substrate contained a double-stranded break without any cohesive overhang and included 10%(w/v) PEG 3350. Details of the assay conditions are given in Section S2. Values represent the mean of duplicate measurements and error bars represent the standard deviation from the mean.

Journal logoSTRUCTURAL BIOLOGY
COMMUNICATIONS
ISSN: 2053-230X
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