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Figure 3
DNA-ligation activity of different DNA breaks by T4-like DNA ligase enzymes. The Nick substrate contained a break on a single strand of the DNA duplex. The overhang substrate contained a cohesive double-stranded break with a four base-pair overhang, the TA + PEG substrate contained a cohesive double-stranded break with a single T–A overhang and included 10%(w/v) PEG 3350, and the Blunt + PEG substrate contained a double-stranded break without any cohesive overhang and included 10%(w/v) PEG 3350. Details of the assay conditions are given in Section S2. Values represent the mean of duplicate measurements and error bars represent the standard deviation from the mean. |

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