Preparing for successful protein crystallization experiments

All crystal-based structural biology methods, including X-ray crystallography, serial synchrotron and serial femtosecond crystallography, and electron diffraction, require the preparation of biomolecular crystals. This article covers strategies for careful sample preparation in crystallization experiments to increase the chance of success.

The structure of a family 168 glycoside hydrolase from the marine bacterium Muricauda eckloniae

The structure of a putative fucoidan-degrading glycoside hydrolase assigned to glycoside hydrolase family 168 reveals a (β/α)₈ fold. The catalytic machinery and potential substrate specificity were investigated through a structural comparison with a Fun168A oligosaccharide complex.

Functional and structural characterization of Stenotrophomonas maltophilia EntB, an unusual form of isochorismatase for siderophore synthesis

The crystal structure of S. maltophilia EntB revealed an unusual form of isochorismate lyase that is involved in siderophore biosynthesis.

Cryo-EM structures of Mycobacterium tuberculosis imidazole glycerol phosphate dehydratase in the apo state and in the presence of small molecules

Cryo-EM structures of M. tuberculosis imidazole glycerol phosphate dehydratase suggest that the enzyme exhibits a 24-mer assembly with 432 symmetry in both apo and small-molecule-bound states.

Crystal structure of the sucrose phosphorylase from Alteromonas mediterranea shows a loop transition in the active site

Sucrose phosphorylases catalyse a bi-bi reaction that interconverts sucrose and phosphate into glucose α-1-phosphate and fructose. Here, we present the first crystal structure of a sucrose phosphorylase from a marine organism.

Crystal structure analysis of oxygen-induced degradation occurring in rsCherry

Four crystal structures of rsCherry, where `rs' means reversible switchable, degraded under aerobic conditions provide valuable insights into oxygen-induced degradation in red fluorescent proteins.

Structural insights into full-length human fascin1: a target for cancer treatment

This study presents the first fully resolved crystal structure of human fascin1, revealing its conformational plasticity and key interdomain interactions critical for actin bundling and cancer metastasis. These findings provide a structural foundation for the development of allosteric inhibitors and pave the way for time-resolved crystallographic studies to explore fascin1 dynamics in metastasis and drug targeting.