Crystal structure of a short-chain de­hydrogenase from Brucella ovis with apo and coenzyme NAD⁺-bound protomer chains

This article describes the structural characterization of a short-chain dehydrogenase from the pathogenic bacterium Brucella ovis. In this manuscript, two structures are described: the structure of the apo protein and a structure containing the coenzyme NAD⁺.

Structural analysis of the tetra­hydrobiopterin glu­cosyltransferase PsBGluT from Pseudanabaena sp. Chao 1811

This study advances our understanding of pteridine glycosylation and also provides a structural basis for investigating photosynthetic signaling pathways in cyanobacteria.

Crystal structures of PF1765 from Pyrococcus furiosus from several different crystallization conditions with varied pH, salt and precipitant

PF1765 from P. furiosus crystallized from a single protein batch in 104 of 192 conditions, yielding ten high-resolution structures (1.1–1.5 Å) across two space groups. Despite wide chemical variability, the overall fold remained nearly identical, with conserved crystal contacts and hydration sites, while subtle lattice-specific rotameric differences highlight the utility of this protein as a model for studying packing effects and microheterogeneity.

CryoSift: an accessible and automated CNN-driven tool for cryo-EM 2D class selection

CryoSift is a CNN-based tool that assesses the quality of 2D class averages. The incorporation of CryoSift into cryoSPARC workflows enables automated particle-stack cleaning.