Crystal structure of Legionella pneumophila glycosidase effector LegY

We report the 1.46 Å resolution crystal structure of the L. pneumophila GH15 glycosidase effector LegY, a single-domain protein with a canonical (α/α)₆-barrel catalytic domain that is structurally and phylogenetically highly similar to fungal glucoamylases, and identify its conserved catalytic residues Glu206 and Glu405 that support a GH15-characteristic single-displacement catalytic mechanism; these findings provide pioneering structural and evolutionary insights into LegY, laying a foundation for understanding its role in L. pneumophila infection and the evolutionary dynamics of GH15 family enzymes.

WebCalEM: a browser-based tool for routine and accurate pixel-size calibration in cryo-EM

WebCalEM is a browser-based, install-free application that performs routine cryo-EM pixel-size calibration directly from gold or graphene oxide reflections in standard sample-support grids using subpixel Fourier-space peak localization; it reproduces the precision of established command-line calibration tools while removing the installation barrier and supporting retrospective per-region calibration on archived datasets.