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The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology Communications.
See also Forthcoming articles in all IUCr journals.
research papersComputer-controlled liquid-nitrogen drizzling device for removing frost from cryopreserved crystals
Abstract Cryocrystallography commonly used in macromolecular crystallography may sometimes reduce the quality of diffraction data and the visibility of crystals due to frost adhesion. We developed a device to remove frost by drizzling liquid nitrogen on the crystals, which enabling noise reduction of diffraction images and crystal-centering with low visibility due to frost adhesion.
research communications (RC)Chitoporin from Serratia marcescens: recombinant expression, purification and crystallization
Serratia marcescens is an opportunistic pathogen that can use chitin-enriched nutrients as an alternative energy source. We identified a chitoporin (ChiP), termed SmChiP, from the outer membrane of S. marcescens and report its crystallization without and with chitohexaose. Initial assessment of the complex structure revealed electron density for the sugar ligand.
research communications (RC)The A component (SmhA) of a tripartite pore-forming toxin from Serratia marcescens: expression, purification and crystallographic analysis
The crystallization of a selenomethionine derivative of the A component of the tripartite haemolytic pore-forming toxin from S. marcescens is reported.
research communications (RC)Crystal structure of the GDP-bound GTPase domain of Rab5a from Leishmania donovani
L. donovani Rab5a (LdRab5a), a GTP-dependent molecular switch, regulates the fluid-phase endocytosis of essential nutrients. The crystal structure of the GTPase domain of LdRab5a has been determined in the GDP-bound form, which shows the canonical Rab fold but with a unique disposition of the Switch I region.
research communications (RC)Structural characterization of a nonhydrolyzing UDP-GlcNAc 2-epimerase from Neisseria meningitidis serogroup A
Crystal structures of a bacterial nonhydrolyzing UDP-GlcNAc 2-epimerase with and without substrates are described. The structures, when compared with homologous enzymes, shed additional light on the mechanism and understanding of the non-allosteric nature of the enzyme.
