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The following articles are a selection of those recently accepted for publication in Acta Crystallographica Section F: Structural Biology Communications.
See also Forthcoming articles in all IUCr journals.
research communications (RC)Recombinant versus native botulinum neurotoxin type A: structural and pharmacodynamic comparison
For the first time, the recombinant core BoNT/A expressed in E. coli and nicked in vitro is shown to be structurally identical to native BoNT/A from C. botulinum, enabling direct activity comparison between the recombinant product and the native toxin.
research communications (RC)Crystal structure of the bifunctional enzyme phosphoribosyl isomerase A from Mycobacterium tuberculosis with bound light-controllable inhibitor
We have determined the crystal structure of an isomerase catalyzing two steps in histidine and tryptophan biosynthesis with a bound light-switchable competitive inhibitor. The work identifies explains the mode of action of the inhibitor and describes the discovery of an hitherto unknown third phosphate-binding site of the enzyme.
research communications (RC)Structure of recombinant Thermoascus aurantiacus xylanase TaXyn10 with an authentic N-terminal pyroglutamate
The high-resolution structure of the T. aurantiacus xylanase TaXyn10 expressed in E. coli shows that recombinant production combined with split-intein-based purification recovers the native fold of TaXyn10 and permits the formation of an N-terminal pyroglutamate. These results demonstrate that this approach can enable recombinant proteins to adopt their native mature state in E. coli, including physiologically relevant post-translational modifications such as N-terminal pyroglutamate formation.
research communications (RC)