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Figure 5
Atomic resolution cryo-EM reconstruction of the 200 kV apoferritin dataset. (a) Overview of the 3D density map of a monomer. (b) Plot of the gold-standard FSC (GS FSC), indicating a 1.24 Å resolution at the 0.143 level and the map-model FSC indicating a 1.25 Å resolution at the 0.5 level. (c) Side-chain map features. The grey surface is at a lower threshold level typically used to depict the map. The blue surface is at a high threshold level, showcasing the atomic resolution of the map with blobs for individual atoms. The green surface represents an Fo − Fc difference density map (3σ level, normalized within a mask) calculated from a hy­dro­gen-omit model. Positive density peaks are clearly visible at positions corresponding to H atoms in the mol­ecular model.

IUCrJ
Volume 13| Part 4| July 2026|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526004100