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Figure 7
Effect of radiation damage on resolution and map detail. (a) Plot of the resolution of reconstructions from the first 1000 movies containing 58k particles versus pre-exposure (red symbols), emulated by omitting a varying number of initial movie frames. The experimental distribution was well fit (R2 = 0.997) by a parabola (red line, coefficients in the legend). The plot also contains numerical estimates of the effect of radiation damage using the measured (black symbols) and 82% of the measured (blue symbols) total exposure. (b) Pre-exposure map series illustrating the effect of radiation damage on side-chain detail. Acidic residues (Glu and Asp) are deca­rboxylated and fade faster than large aromatic residues. The pre-exposure value and map resolution are shown in the top left corner of each panel.

IUCrJ
Volume 13| Part 4| July 2026|
ISSN: 2052-2525
https://doi.org/10.1107/S2052252526004100