Crystallization and preliminary crystallographic analysis of β-L-arabinopyranosidase from Streptomyces avermitilis NBRC14893

β-L-Arabinopyranosidase from Streptomyces avermitilis NBRC14893 is a monomeric protein consisting of a catalytic domain belonging to glycosyl hydrolase family 27, an unknown domain and a substrate-binding domain belonging to carbohydrate-binding module family 13. The complete enzyme (residues 45–658) has successfully been cloned and homologously expressed in the Streptomyces expression system. β-L-Arabinopyranosidase was crystallized by the sitting-drop vapour-diffusion method. The crystals diffracted to 1.6 Å resolution and belonged to space group P2₁2₁2₁, with unit-cell parameters a = 68.2, b = 98.9, c = 181.3 Å. The Matthews coefficient was calculated to be 2.38 ų Da⁻¹.