Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597

Sugiyama, Y.; Senda, M.; Senda, T.; Matsuda, T.

doi:10.1107/S2053230X15002265

Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597

Y. Sugiyama, M. Senda, T. Senda and T. Matsuda

Acetophenone reductase (APRD) from Geotrichum candidium NBRC 4597 was crystallized by the hanging-drop vapour-diffusion method using PEG 3350 as a precipitant. The crystal belonged to space group P6₅22, with unit-cell parameters a = b = 104.5, c = 273.7 Å, and diffracted to 2.6 Å resolution. Phasing using the single-wavelength anomalous diffraction method was successful. Model building and crystallographic refinement are in progress.

Keywords: acetophenone reductase; enantioselectivity; asymmetric reduction.

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Crystallization and preliminary crystallographic analysis of acetophenone reductase from Geotrichum candidum NBRC 4597

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	Portable Network Graphics (PNG) image https://doi.org/10.1107/S2053230X15002265/nw5017sup1.png Figure S1. Anomalous difference Patterson map of the section z = 0.5 at 3.5 Å resolution. Contour levels from 3σ to 10σ are drawn in a 0.5σ steps.
	Portable Network Graphics (PNG) image https://doi.org/10.1107/S2053230X15002265/nw5017sup2.png Figure S.2 An electron-density map by SAD phasing using the program autoSHARP. The electron density was contoured at the level of 1σ. A polyalanine model from AutoSHARP is also shown.