Figure 2
(a) DLS instrumentation attached to the SAXS SEU at beamline P12. The flange-mounted DLS laser and detection optics and fibres are located on opposite sides of the SEU. (b) Close-up image of the incident laser beam passing the sample solution inside the SAXS SEU (top view). Scattered light in the direction of the detectors is symbolized by four red dashed lines. (c) Software window and a representative four-channel DLS measurement of BSA. The four data collection channels (ACF, count rate and histogram representation) are colour-coded in blue, red, green and yellow in the order from 1 to 4. (d) Histogram presentation of the hydrodynamic radius distribution, weighted by scattering, obtained for 9 mg ml−1 BSA solution in PBS. The hydrodynamic radius with the highest abundance in the averaged measurements is listed for each channel along with the PDI, derived from the mean peak broadness resulting from CONTIN analysis. The molecular weight is estimated according to Cantor & Schimmel (1980), assuming a globular shape of the particle. Moreover, the abundancy of a radius value peak in the set of measurements (N) and the relative size of the peak area (A) are specified. |