Figure 1
(a) Simulated soft X-ray projection and (b)–(d) fluorescence images with random fluorescence spheres. The small images at the bottom of (a) and (b) show situations in which a few fluorescence spheres agglomerate in the soft X-ray projection and fluorescence image. (b) Fluorescence spheres and (c)–(d) stained structures indicated by red arrows are in the focal planes. Scale bars: (a)–(d): 2 µm; small images at the bottom of (a) and (b): 500 nm. |