Figure 9
Correlative imaging of mitochondria of the mast cell. (a) Fluorescence image of the protuberance part of the mast cell, (b) mosaic image of soft X-ray projections at 0° of the same part of the mast cell and (c) fused image of (a) and (b). An enlargement of the area surrounded by the red square in (c) is shown in (d). (e) Fused image of the fluorescence image and a slice of the soft X-ray tomography image. (f) Segmentations of mitochondria. (g) Enlargements of the segmentations of mitochondria and corresponding soft X-ray tomography slices. The different colors in (c), (d) and (e) indicate the intensity of normalized fluorescence. Scale bars: (a), (b), (c): 10 µm; (d), (e), (f): 1 µm; (g): 500 nm. |