Nanoscopic X-ray imaging and quantification of the iron cellular architecture within single fibroblasts of Friedreich's ataxia patients

De Samber, B.; Vanden Berghe, T.; Meul, E.; Bauters, S.; Seyrich, M.; Smet, J.; De Paepe, B.; Silva, J. C. da; Bohic, S.; Cloetens, P.; Van Coster, R.; Vandenabeele, P.; Vincze, L.

doi:10.1107/S1600577519015510

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Figure 3
Overview of different iron-rich, (sub)micrometre structures found in other freeze-dried FRDA fibroblasts. (a), (b), (c) Iron-rich structures found within FRDA fibroblasts cultured upon the same silicon nitride membrane and freeze-dried using a commercial freeze-drying instrument. (d) Enlargement of the iron-rich structure discussed in Fig. 2(h)

. (e) Iron-rich structure found back in a cryogenically frozen fibroblast (also at ID16A beamline, but in cryo-mode). All areal elemental concentrations of iron were background-corrected and are provided as `No. of atoms/pixel'; quantification for iron was based upon an AXO thin film standard. For quantification of the cryofrozen fibroblast, the K-K_α/K_β ratio of the entire map was used for estimating the approximate ice thickness covering the sample. White dashed lines indicate the area that was used for determining the surface area and the iron areal concentration. For each elemental map, step size (Δ) and dwell time (t) are indicated.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 27| Part 1| January 2020| Pages 185-198

https://doi.org/10.1107/S1600577519015510

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