Nanoscopic X-ray imaging and quantification of the iron cellular architecture within single fibroblasts of Friedreich's ataxia patients

De Samber, B.; Vanden Berghe, T.; Meul, E.; Bauters, S.; Seyrich, M.; Smet, J.; De Paepe, B.; Silva, J. C. da; Bohic, S.; Cloetens, P.; Van Coster, R.; Vandenabeele, P.; Vincze, L.

doi:10.1107/S1600577519015510

Figure 4
(Upper images) Elemental distributions of potassium, iron and sulfur obtained upon a freeze-dried FRDA fibroblast. All maps were scanned with 50 ms dwell time and have a pixel size of 50 nm. Several iron-rich regions were detected, marked with red rectangles and denoted `a' to `g'. For clarity, cell boundaries are marked with a white dashed line. For better scaling, areal elemental concentrations of potassium are provided in (ng cm⁻²)^1/2, areal elemental concentrations of iron are provided in [(No. of atoms)/pixel]^1/2. (Lower images) High resolution (20 nm or 10 nm pixel size) of iron elemental distributions of the iron-rich hot-spots detected. All areal concentrations are background-corrected. The asterisks (*) indicates regions that were used for background subtraction. Areal elemental concentrations of iron are provided in `No. of atoms/pixel'. White dashed lines indicate the area which was used for determining the surface area and the iron areal concentration. For each elemental map, step size (Δ) and dwell time (t) are indicated. Quantification for iron was based upon an AXO thin film standard and upon NIST SRM 1577C for all other elements.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 27| Part 1| January 2020| Pages 185-198

https://doi.org/10.1107/S1600577519015510

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