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Figure 2
X-ray phase contrast tomography images of the lumbar region of the spinal cord. Reconstructed volumes (about 1 mm thick) of the lumbar region of the spinal cord. Axial sections of healthy (a), pre-symptomatic (b) and 90 days ALS affected (c) samples. Cell loss induced by ALS progression can be observed in (b) and (c). Lower inset (green): magnification of ROIs in the ventral horn of the spinal cord containing neuronal cells compatible with motor neurons. Upper insets (yellow): magnification of ROIs to show neuronal cells compatible with sensor interneurons. In a pre-symptomatic stage, the number of the sensory neurons remains almost unaltered compared with the control sample, while a decrease in cell number is shown in the 90 days SOD1 mice. Images are obtained with a pixel size of 3.06 µm at ID17 at the ESRF. (d) Schematic representation of the selected ROIs shown in (a, b, c): quantitative analysis was performed in the ventral horn (green) of the spinal cord. (e) Percentage of the number of neurons in ALS samples compared with the control samples in the ventral horn at different time-points. Error bars represent standard deviation of data.

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