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Figure 2
Dark-field contrast image segmentation. (a) X-ray dark-field contrast image for the complete scan of the entire window. Scale bar: 200 µm. (b) Detail of the region inside the white box in (a). (c) Result of local thresholding, showing the same region as in (b), but the pixels identified as background have been masked out. The contours of the mask are shown in white. (d) Result of global thresholding on single cells, showing the same region as in (b), but only the nuclei. The contours of the cell bodies are shown in white. (e) Final regions of interest for the region shown in (b). Background is shown in blue, cytoplasm in green and nuclei in red. Black pixels are disregarded. The color scales have been readjusted individually in (a)–(d) for better visualization.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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