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Figure 4
Analysis of different data ensembles. (a) ROIs, entire window analysis. Background is shown in blue, cytoplasm in green and nuclei in red. (b) ROIs, cell-by-cell analysis. Background is shown in black, cytoplasm in teal and nucleus in orange. (c) Enlargement of the region in the red box in (b): example of ROIs for one cell. (d) Dark-field contrast image for the cell shown in (c). The positions of the scattering patterns used to exemplify single scattering pattern analysis [see (e), (f), (g)] are marked by crosses in yellow for the cytoplasm and pink for the nucleus. (e) Radial intensity profiles for the cytoplasmic and nuclear regions, after background subtraction. The curves were obtained by azimuthal integration of average scattering patterns coming from different ensembles: averaged over the entire window [ROIs shown in (a)], averaged over an individual cell [ROIs shown in (c)] or single (not averaged) scattering patterns [marked in d)]. (f) Distribution of K values obtained from the cell subset shown in (b). The values obtained from the entire window analysis and from the single scattering pattern analysis are also shown. (g) Distribution of α values obtained from the cell subset shown in (b). The values obtained from the entire window analysis and from the single scattering pattern analysis are also shown. For each bin in (f) and (g), the frequencies are obtained by dividing the counts by the total number of values and then normalized by dividing them by the width of the bin.

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SYNCHROTRON
RADIATION
ISSN: 1600-5775
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