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Figure 4
(a–d) Background-corrected holograms of representative living, as well as fixed and stained cells for 9.9 keV (a, b) and 13.8 keV (c, d). The dashed lines represent the outline of the reconstruction support (see Fig. S2 in the supporting information for a version without the dashed lines). The exposure time for the holograms is 30 s. Black dots in the holograms and reconstructions represent BaSO4 grains (see black filled arrows for examples) that are attached to cells. Scale bars: 10 µm. (e–h) Reconstructed phase shift caused by the holograms in (a–d). A CTF reconstruction is used as initial phase guess which is refined using the RAAR algorithm with 500 iterations. To improve visibility of the reconstructed nucleus and cytoplasm, the phase shift from BaSO4 grains goes beyond the chosen color scale of these plots. (e) The cell nucleus is visible in some reconstructions of living cells that are recorded at 9.9 keV (see black open arrow). The spatial axes are given in pixels (px).

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 31| Part 4| July 2024| Pages 923-935
https://doi.org/10.1107/S1600577524003618
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