Shedding light on biochemical changes in single neuron-like pheochromocytoma cells following exposure to synchrotron sourced terahertz radiation using synchrotron source Fourier transform infrared microspectroscopy

Perera, P.G.T.; Vongsvivut, J.; Linklater, D.; Vilagosh, Z.; Appadoo, D.; Nguyen, T.H.P.; Tobin, M.J.; Croft, R.; Ivanova, E.P.

doi:10.1107/S1600577524010944

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Figure 1
Experimental design and the technical setup at the Australian Synchrotron IRM beamline. (a) Following 10 min of SS THz exposures, the PC 12 cells were analysed for morphological changes at the membrane level including cell blebbing and membrane permeability. (b) Principle behind FTIR microspectroscopy: a beam consisting of IR wavelengths is sent through a beam splitter, where it is reflected via the mirrors and recombined to construct an interference pattern which is then sent to the sample and the transmitted portion reaches the detector. Fourier transformation is then performed to obtain a full spectrum as a function of wavenumbers. (c) FTIR microscope and spectrometer used on the IRM beamline at the Australian Synchrotron

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 32| Part 1| January 2025|

https://doi.org/10.1107/S1600577524010944

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