High-resolution imaging of organic and inorganic nanoparticles at nanometre-scale resolution by X-ray ensemble diffraction microscopy

Chen, N.-J.; Yeh, C.-H.; Cao, H.-Y.; Chen, N.-C.; Chen, C.-J.; Chen, C.-Y.; Tsai, Y.-W.; Lin, J.-M.; Huang, Y.-S.; Hsiao, C.-N.; Chen, C.-C.

doi:10.1107/S1600577524010567

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Figure 8
The calibration of the reconstructed NV-LP sample at a radial resolution of 1.3 nm per pixel. (a) The analyzed final diffraction pattern from a total of 85616 patterns and (b) the image retrieved by the guided hybrid input–output method. (c) An approximate rotation-averaged image of nodavirus generated by adding together cryo-EM images of 14 classifications. (d) A rotation-averaged image of the NV-LP model generated by summing all possible orientations with 1° increments. (e) A line-profile comparison of the central columns in panels (b)–(d). The full width at half maximum (labeled by double arrows) of the sharpest profile demonstrates a high consistency of the shell thickness across currently existing approaches: crystallography (red), cryo-EM (black) and our approaches (blue). (f) An overlay of the overall protein structure and the reconstructed image on the same scale depicts the density distribution of the hollow structure observed in both cases.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 32| Part 1| January 2025|

https://doi.org/10.1107/S1600577524010567

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