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Figure 6
Residue-specific speed of solvent accessibility change. The crystal structure of SpyCatcher003 (gray) was extended to include additional C-terminus residues by homology modeling using PDB entry 2x5p (Oke et al., 2010BB101), the original fibronectin binding protein from which SpyCatcher was derived. Residues in light blue and red indicate a slow rate of decrease and increase, respectively; residues in deep blue indicate a fast rate of decrease; residues in gray did not show any change in solvent accessibility during binding of SpyTag003 to SpyCatcher003. SpyTag003 is shown in black. The location of the attached fluorescence molecules, sfGFP and Alexa 555, attached to the C-terminal of SpyTag003 and Cys49 of SpyCatcher003, respectively, are shown in green and pink.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
Volume 32| Part 4| July 2025|
https://doi.org/10.1107/S1600577525004205
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