ALS-ENABLE: creating synergy and opportunity at the Advanced Light Source synchrotron structural biology beamlines

Ralston, C.Y.; Gupta, S.; del Mundo, J.T.; Soe, A.C.; Russell, B.; Rad, B.; Tyler, J.; Paul, S.; Kahan, D.N.; Kristensen, L.G.; Subramanian, S.; Kidd, S.; Burnett, K.; Sankaran, B.; Classen, S.; Progozhin, D.; Taylor, J.R.; Dickert, J.M.; Royal, K.B.; Rozales, A.; Ortega, S.L.; Allaire, M.; Nix, J.C.; Hura, G.L.; Holton, J.M.; Hammel, M.; Adams, P.D.

doi:10.1107/S1600577525004205

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Figure 7
Differences in electrostatic potential maps. The SpyTag001 and SpyTag003 structures superimposed on the electrostatic potential map of the homology-modeled extended sequence of SpyCatcher001 (left) and SpyCatcher003 (right), respectively. The three mutations, Q97D, K105E and K108E, make a significant surface polarity switch towards the N-terminal section of SpyTag003.

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Volume 32| Part 4| July 2025|

https://doi.org/10.1107/S1600577525004205

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