AF4-to-SAXS: expanded characterization of nanoparticles and proteins at the P12 BioSAXS beamline

Da Vela, S.; Bartels, K.; Franke, D.; Soloviov, D.; Gräwert, T.; Molodenskiy, D.; Kolb, B.; Wilhelmy, C.; Drexel, R.; Meier, F.; Haas, H.; Langguth, P.; Graewert, M.A.

doi:10.1107/S1600577525003959

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Figure 2
Workflow and software integration. (a) Schematic overview depicting the integration of the AF4 system and NovaFFF software into the SAXS beamline control system, BECQUEREL. The workflow illustrates the information flow from the beamline operator through input fields to data collection. The beamline meta server sends a command to the automated sample loader for sample injection. Once the injection is confirmed, SAXS data frames are recorded while NovaFFF regulates the flow according to the programmed separation protocol and simultaneously records UV and MALS data. (b) Screenshot of the BECQUEREL graphical user interface (GUI) in AF4 mode. (c) List of selectable input parameters provided in BECQUEREL for the beamline operator to configure experiments.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 32| Part 4| July 2025| Pages 971-985

https://doi.org/10.1107/S1600577525003959

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