Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections

Roter, B.; Crawford, A.M.; Jin, Q.; Glowacki, A.T.; Lai, B.; Marin, F.S.; Maxey, E.; Shi, X.; Culotta, V.C.; Wildeman, A.S.; Patel, N.K.; O'Halloran, T.V.; Jacobsen, C.

doi:10.1107/S1600577526000925

Figure 1
Scanning fluorescence X-ray microscopy (SFXM) images elemental distributions at different length scales. Shown here are trace essential metals inside a sample of a mouse kidney partial section infected with Candida albicans, with Ca being strongly elevated at sites of infection. We obtained the images in (a) using the KB mirror scanning station at 8-BM with a 25 µm step size at t_dwell = 50 ms. These metals are also displayed in a color composite map, allowing one to more easily see how the elements are differentially distributed in the specimen. The images in (b) and (c) correspond to regions we selected from (a) using the KB mirror and capillary optics, respectively. In (b), we used a Δ_x = Δ_y = 5 µm step size at t_dwell = 100 ms. In (c), we used step sizes of Δ_x = 1 µm and Δ_y = 2 µm in y. The spatial resolution improved from 10.5 µm to 6.3 µm when transitioning from the KB optic to the capillary (as described in Section 2.2

). The large-scanning area SFXM capabilities of 8-BM described in this work provide an important complement to nanoscale imaging capabilities from other beamlines at the Advanced Photon Source at Argonne National Laboratory.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 33| Part 2| March 2026| Pages 319-330

https://doi.org/10.1107/S1600577526000925

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