Multifunctional bending magnet beamline with a capillary optic for X-ray fluorescence studies of metals in tissue sections

Roter, B.; Crawford, A.M.; Jin, Q.; Glowacki, A.T.; Lai, B.; Marin, F.S.; Maxey, E.; Shi, X.; Culotta, V.C.; Wildeman, A.S.; Patel, N.K.; O'Halloran, T.V.; Jacobsen, C.

doi:10.1107/S1600577526000925

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Figure 5
Image of a Candida albicans-infected mouse kidney partial section obtained using the capillary optic scan station and a per-pixel scan time of t_dwell = 50 ms. At left (a) is the calcium fluorescence image from Fig. 1

(c) as scanned, which is rotated relative to the orientation shown in that figure. At right (b) are power spectral density plots for the images of three different elements obtained from the same scan: Ca, Fe, and Zn. Because of differences in concentrations of each of these elements in the sample and differing fluorescence yields and background signals, each element's image and power spectral density had different signal and noise characteristics. As described in Section 2.1

, this leads to different values for the `knee' spatial frequency u_knee of equation (12)

with spatial half-period δ_knee = 1/(2u_knee), as well as the spatial frequency u_res of equation (13)

and corresponding spatial resolution δ_res of equation (14)

for a signal-to-noise ratio of SNR_res = 5.

JOURNAL OF
SYNCHROTRON
RADIATION

ISSN: 1600-5775

Volume 33| Part 2| March 2026| Pages 319-330

https://doi.org/10.1107/S1600577526000925

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