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Figure 6
(a) The distribution over pixels in the samples of the collagen D-period calculated from fifth- and sixth-order peak positions, for non-zero meridional intensity collagen scan points in all samples, is shown using box plots. Individual sample labels along the x axis contain two box plots each, with the left one corresponding to the D-period calculated from the fifth peak and the right plot calculated from the sixth peak. (b) The corresponding FWHM distribution of the fifth (left) and sixth (right) peaks are displayed in the same order. Statistical significance was tested against the underlined samples (colour-coded) using Wilcoxon signed-rank test (`ns'/not significant → P > 0.05; `*' → P ≤ 0.05; `**' → P ≤ 0.01; `***' → P ≤ 0.001). Parameters of samples from silica beads to paraffin(I) were statistically tested against the unprocessed sample; PBS (wet) is additionally tested against PBS (dry), while the −80°C-preserved sample is tested against a −80°C+OCT-preserved sample. The paraffin(II)-embedded sample was statistically tested against the unprocessed(II) sample. The Technovit 9100-embedded sample was excluded from this analysis due to non-existent collagen peaks. |

journal menu![[Figure 6]](bon5001fig6.jpg)
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