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Figure 1
Experimental setup. (a) Schematic side view of the dedicated flow chamber (see legend for the individual components). Two SiN membranes are separated by 20 µm using SU-8 spacers. This `sandwich' is enclosed by PDMS layers and clamped by metal frames. The buffer flow is represented by blue dashed arrows. (b) Schematic drawing of the SAXS experiment (not to scale). The sample (fixed-hydrated cells on a SiN membrane) is scanned through the focused X-rays. The beamstop blocks the direct beam, and the two-dimensional detector records the scattered X-rays. The red dashed lines represent the scattering angle 2θ between the incoming beam direction and the scattered X-rays. (c) The flow within the microfluidic chamber is oriented horizontally in perpendicular direction to the incoming beam, thus along the y axis. (d) Representation of the scanning scheme; the SiN membrane is moved along the y and z axes to obtain a raster scan of the selected region with the specified step numbers and sizes.

Journal logoJOURNAL OF
SYNCHROTRON
RADIATION
ISSN: 1600-5775
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