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![[Figure 4]](vl5055fig4mag.jpg)
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Figure 4
Analysis of radiation damage. (a) First (left), second (center) and fifth (right) dark-field contrast images obtained from multiple scans of the same region. Each cell in the first scan is labeled individually, represented by different colors in Fig. S7. Scale bars: 20 µm. (b) Anisotropy distribution of a single example cell that is labeled in green in panel (a) plotted against the scan number. In the cytoplasm region of individually labeled cells, while excluding the nuclear region, only pixels with anisotropy values greater than the 99th percentile of the anisotropy distribution of the background region are used. The horizontal lines in each violin plot represent the 25%, 50% and 75% quartiles from the bottom to the top, and the asterisks denote the means; the 50% quartile corresponds to the median. (c) Degree of anisotropy decay from the first to the second scan of individual cells using different measurement conditions (step size and exposure times, see legend above the figure). The color of each symbol corresponds to the specified buffer flow rate. Darker symbols show the mean values for each respective condition. |
![[Figure 4]](vl5055fig4.jpg)
 | JOURNAL OF SYNCHROTRON RADIATION |
ISSN: 1600-5775
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