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Figure 2
Ultrastructural preservation following critical point drying and post-CPD embedding in resin. (a) Divergent preparation workflows for TEM: tissue is either embedded directly in resin (top) or dried using CPD and subsequently embedded (bottom) prior to ultramicrotomy and TEM imaging. (b) TEM images of standard resin-embedded tissue show well preserved ultrastructure, including clear nuclear membranes, myelinated axons, and synaptic vesicle populations. (c) CPD-treated tissue post embedded in resin retains qualitatively comparable morphological fidelity, with intact subcellular features throughout. The anatomical locations of the TEM sections used in this figure are shown in Fig. S4, and additional representative TEM images from independent regions are shown in Fig. S5. |

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